polyclonal rabbit anti human ido1  (Danaher Inc)

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: TDO, IDO1 and AhR expression in HUVEC and ECFC. ( A ) Real-time PCR analysis (mean ± SEM, n = 3). ( B , C ) Immunofluorescence, mean ± SEM, n = 3 and representative photomicrographs at 40× magnification for TDO (red) and IDO1 (green). ( D , E ) AhR (green) localization in the nucleus following the pro-angiogenic stimulus VEGF-A. Histograms show AhR fluorescence nuclear localization (AhR/DAPI) by Mander’s coefficient (M1) using Image J 1.54d software.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Fluorescence, Software

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of TDO and IDO1 inhibitors on in vitro angiogenesis. Angiogenesis was measured by capillary morphogenesis at 6 h in untreated HUVECs (ctr) and cells stimulated with 20 ng/mL VEGF-A in the absence or presence of 680C91 and epacadostat. ( A ) Representative pictures, 10× magnification. ( B ) Mean ± SEM, n = 3. § p < 0.05; §§ p < 0.04; §§§ p < 0.001 vs. untreated (ctr). *** p < 0.001 vs. VEGF-A.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: In Vitro

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of TDO and IDO1 inhibitors on in vitro vasculogenesis. Vasculogenesis was measured by capillary morphogenesis at 24 h in untreated ECFCs (ctr) and cells stimulated with 20 ng/mL VEGF-A in the absence or presence of 680C91 and epacadostat. ( A ) Representative pictures. 10× magnification. ( B ) Mean ± SEM, n = 3; § p < 0.05; §§ p < 0.01 vs. untreated (ctr). * p < 0.05; ** p < 0.01; *** p < 0.001 vs. VEGF-A.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: In Vitro

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of the TDO inhibitor 680C31 and IDO1 inhibitor epacadostat on HUVEC proliferation. Cell proliferation was evaluated in response to ( A , C ) VEGF-A and ( B , D ) FGF-2 in the presence of increasing concentrations of 680C91 ( A , B ). ( C , D ) Effects of the highest concentration of 680C91 (40 µM) and epacadostat (1 µM) on VEGF-A- and FGF-2-induced HUVEC growth. Mean ± SEM, n = 5; * p < 0.05 vs. VEGF-A alone; ** p < 0.01 vs. FGF-2 alone.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Concentration Assay

Journal: Journal of personalized medicine

Article Title: IDO1/COX2 Expression Is Associated with Poor Prognosis in Colorectal Cancer Liver Oligometastases.

doi: 10.3390/jpm13030496

Figure Lengend Snippet: Figure 1. IDO1, COX2, CD3 and CD8 expression levels in CRC patients with liver oligometas- tases. (a) Immunohistochemistry (IHC) of negative IDO1 expression in CRC liver oligometastases; (b) immunohistochemistry (IHC) of positive IDO1 expression in CRC patients with liver oligometas- tases; (c) immunohistochemistry (IHC) of negative COX2 expression in CRC liver oligometastases; (d) immunohistochemistry (IHC) of positive COX2 expression in CRC liver oligometastases; (e) im- munohistochemistry (IHC) of positive CD3 expression in CRC liver oligometastases; (f) immunohis- tochemistry (IHC) of positive CD8 expression in CRC liver oligometastases.

Article Snippet: 2023, 13, 496 3 of 10 (working dilution, 1:100; Beijing Golden Bridge Biotechnology) and rabbit anti-human IDO1 monoclonal antibody (working dilution: 1:50; Beijing Golden Bridge Biotechnology) overnight at 4 ◦C.

Techniques: Expressing, Immunohistochemistry

Journal: Journal of personalized medicine

Article Title: IDO1/COX2 Expression Is Associated with Poor Prognosis in Colorectal Cancer Liver Oligometastases.

doi: 10.3390/jpm13030496

Figure Lengend Snippet: Figure 2. Correlation of the clinicopathological parameters with OS in CRC patients with liver oligometastases. Survival curves were generated using the Kaplan–Meier method, and differences between survival curves were estimated using the log-rank test. (a) We divided all patients into four groups based on the level of IDO1 and COX2 expression. Group I: IDO1LowCOXLow; Group II: IDO1HighCOXLow; Group III: IDO1LowCOXHigh; Group IV: IDO1HighCOXHigh. The association of the four groups (IV vs. I-III) with OS was significant (p = 0.002); (b,c) correlation between CEA and N stage and OS in patients; b: CEA (p = 0.024); c: N stage (p = 0.05). (d–n): Correlation between other clinicopathological parameters and OS in patients. (p > 0.05).

Article Snippet: 2023, 13, 496 3 of 10 (working dilution, 1:100; Beijing Golden Bridge Biotechnology) and rabbit anti-human IDO1 monoclonal antibody (working dilution: 1:50; Beijing Golden Bridge Biotechnology) overnight at 4 ◦C.

Techniques: Generated, Expressing

Journal: Journal of personalized medicine

Article Title: IDO1/COX2 Expression Is Associated with Poor Prognosis in Colorectal Cancer Liver Oligometastases.

doi: 10.3390/jpm13030496

Figure Lengend Snippet: Figure 3. Correlation of the clinicopathological parameters with PFS in CRC patients with liver oligometastases. Survival curves were generated using the Kaplan–Meier method, and differences between survival curves were estimated using the log-rank test. (a) We divided all patients into four groups based on IDO1 and COX2 expression levels. Group I: IDO1LowCOXLow; Group II: IDO1HighCOXLow; Group III: IDO1LowCOXHigh; Group IV: IDO1HighCOXHigh. The association of the four groups (IV vs. I/II/III) with PFS was significant (p = 0.0024); (b,c) correlation between CEA and N stage and PFS in patients; c: N stage (p = 0.026); b: CEA (p = 0.006). (d–n): Correlation between other clinicopathological parameters and PFS in patients. (p > 0.05).

Article Snippet: 2023, 13, 496 3 of 10 (working dilution, 1:100; Beijing Golden Bridge Biotechnology) and rabbit anti-human IDO1 monoclonal antibody (working dilution: 1:50; Beijing Golden Bridge Biotechnology) overnight at 4 ◦C.

Techniques: Generated, Expressing